بررسی بیان ژن های T-bet و Gata-3در بیماران مبتلا به آسم آلرژیک

Background and purpose: Allergic asthma is a chronic inflammatory disease identified by high response to allergens and excessive air ways edema. T-bet and GATA-3 are two transcriptional factors that differentiate Th1 and Th2 from Tnaive. In this study, we examined the expression levels of these two factors in patients with allergic asthma incomparison with healthy controls. Material and methods: In a case- control study, 26 patients with allergic asthma and 26 healthy subjects were studied who were matched for age and sex. Sampling was done and peripheral blood mononuclear cells were isolated and cDNA was synthesized after RNA extraction. Gene’s expressions were evaluated by Real-time Polymerase chain reaction. Results: The results showed that T-bet and GATA-3 expression levels significantly increased in patients in comparison with control group. In addition, GATA-3/T-bet ratio showed a significant increase in case group (P=0.005). Conclusion: This study showed that the genes expression of T-bet and GATA-3 regulate the balance ofTh1/Th2. Therefore, evaluation of T-bet and GATA-3 is believed to have an important role in treatment and clinical condition of asthma patients.